The aim of this research was to determine the prevalence of shoulder pain and its association with three key domains: occupational factors, physical activity and mental health. Both samples had associations between depression or anxiety and shoulder pain reporting, but neither population had associations between physical activity level and shoulder pain. Unfortunately, the physical activity reported did not represent any occupational specific demands performed in the 7-day period. Questionnaires intended to investigate the type and frequency of upper extremity activity could possibly identify an association between physical activity and shoulder pain. We acknowledge a limitation in the collection of pain data, where theGen1 cohort were asked to report shoulder pain experienced in the last month, with Gen2 asked to report if they were currently experiencing shoulder pain.